Electrospinning technology: a promising approach for tendon–bone interface tissue engineering

The regeneration of tendon–bone interface tissue has become a topic of great interest in recent years. However, the complex nature of this interface has posed challenges in finding suitable solutions. Tissue engineering, with its potential to improve clinical outcomes and play a crucial role in musculoskeletal function, has been increasingly explored for tendon–bone interface regeneration. This review focuses on the research advancements of electrospinning technology in interface tissue engineering. By utilizing electrospinning, researchers have been able to fabricate scaffolds with tailored properties to promote the regeneration and integration of tendon and bone tissues. The review discusses the unique structure and function of the tendon–bone interface, the mechanisms involved in its healing, and the limitations currently faced in achieving successful regeneration. Additionally, it highlights the potential of electrospinning technology in scaffold fabrication and its role in facilitating the development of functional and integrated tendon–bone interface tissues. Overall, this review provides valuable insights into the application of electrospinning technology for tendon–bone interface tissue engineering, emphasizing its significance in addressing the challenges associated with regeneration in this complex interface.


Introduction
The musculoskeletal system of the human body functions through the coordinated actions of various tissues, providing support and stability while allowing organized movement of muscles and bones.Ligaments attach bones to bones and tendons connect muscles to bones, making connective tissue an important part of our bodies.The transitional portion from muscle to bone, known as the tendon-bone interface (TBI), is a highly specialized site that can effectively transmit tensile loads from so to hard tissues. 1 These interfaces demonstrate gradient changes in structural, compositional and mechanical properties to efficiently transmit stresses between tendons and bones. 2,3Tears of tendon or ligament insertions are common clinical problems encountered in orthopedic practice.Injuries to the rotator cuff are among the most common sports injuries, usually causing pain, weakness, and limited range of motion in the shoulder, ultimately imposing a heavy nancial burden on families and society. 4Rotator cuff tears restrict shoulder joint movement and severely impact patients' daily lives.Surgical repair is usually needed for rotator cuff tears.While some patients see signicant improvements in shoulder function post-surgery, rerupture rates are still high, ranging from 15-94%. 5,6The high rerupture rate can be attributed to the fact that the injured site is typically located at the tendon-bone interface, a complex structure and composition that makes repair difficult, while scarring causes weak tissues. 7,8Faced with the current situation, rotator cuff surgery remains a challenge and better solutions need to be developed to avoid postoperative reruptures.
Between tendons and bones, there are four layers of structural and compositional transition: tendon, non-mineralized brocartilage, mineralized brocartilage, and bone 9 (Fig. 1).Within the tendon-bone interface, cells and extracellular matrix are arranged in a gradient direction along the interface, with this transitional zone mediating load transfer from tendon to bone and helping transmit forces from the relatively so tendon tissue to the rigid bone, so as to minimize stress concentrations.However, in the healing process from tendon to bone, this unique transitional tissue between tendon and bone is not reconstructed. 5There may be issues with brocartilage regeneration, bone loss, and immune dysregulation due to an imbalance between pro-inammatory and anti-inammatory macrophages aer injury at the bone-tendon interface. 11,12uring the early stages of rotator cuff tears (RCT), pro-inammatory macrophages disproportionately produce interleukin 1 (IL-1), interleukin 6 (IL-6), and tumor necrosis factor (TNF-a), 13 excessive secretion of inammatory factors inhibits brocartilage layer regeneration and enhances osteoclast activity. 12As a result, M2 macrophages do not have sufficient numbers to support bone and brocartilage regeneration during tendon-bone healing. 12Therefore, it is feasible to improve the abnormal inammatory response, promote cartilage regeneration, reduce bone loss and promote osteogenic differentiation in the process of tendon-bone healing. 14,15n recent years, tissue engineering of the tendon-bone interface has gained increasing attention as a potential therapeutic option.For tissue repair, tissue engineering is important because it can mimic natural tissues and provide an extracellular matrix (ECM) environment that mimics natural tissues.ECM microenvironment regulates stem cell behaviors and fates in important ways.With electrospinning, polymer bers can be produced with diameters of 50-1000 nm, which are several orders of magnitude smaller than bers produced with other conventional fabrication methods. 16It has therefore been proposed that electrospinning can produce scaffolds composed of bers that are more similar to natural collagen bers in tendons in terms of their diameter scale and layered structures.There have been numerous studies showing that electrospun nanober scaffolds promote cellular adhesion, growth, proliferation and even differentiation in tendon tissue engineering applications, as well as demonstrating promising regenerative outcomes. 17his review focuses on the tendon-bone interface tissue.First, the current development characteristics of the attachment points of bone tendon and the healing mechanism of the injured interface are discussed.In the following sections, electrospinning technology will be primarily discussed in the context of tendon and bone interface tissue engineering.The second part introduces the design strategy of biomimetic stents prepared by loading active substances, last but not least, it summarizes the eld's potential challenges, as well as future directions.

The structural and development of the tendon-bone interface
Tendon-bone transitions in vivo occur in two forms -brous attachments and brocartilaginous attachments. 18The characteristic of brous attachments is that the tendon-bone interface has dense brous connective tissue, found in tendons and ligaments attaching to the sha or end of long bones (such as the medial collateral ligament, triceps tendon). 19,20Fibrous attachments can be further divided into two types: entheses and osteotendinous junctions.In the former, the tendon is indirectly attached to the bone by the periosteum, while in the latter, it is directly attached to the bone by the tendon. 19In comparison, brocartilaginous attachments occurring at bone protuberances and epicondyles (including the rotator cuff and anterior cruciate ligament) (Fig. 1) are more commonly encountered in human injuries. 19,20This paper will discuss brocartilaginous attachments.Fibrocartilage at bone attachments can be divided into four transitional zones (Fig. 2).A transition occurs between so tissues and hard tissues at these four areas, which contain different collagen, minerals, cells, and other substances.The aim of this transition is to transmit external loads between so tissues and hard tissues, ensuring stresses are minimized as much as possible and promoting joint movement.3][24] Zone II: unmineralized brocartilage zone, avascular and mainly composed of type II and III collagen forming a mesh-like structure.[25][26][27] It delineates zones II and III mechanically by following unmineralized brocartilage and is called the tidemark. 22Zone III: mineralized brocartilage zone, avascular and principally comprised of type II collagen and enlarged brocartilage cells surrounded by proteoglycans, type I and X collagens.This region is highly irregular and represents the true junction between tendon and bone, interfacing with subchondral bone. 21,22,27Zone IV: an osteoclastic, osteocyte, and osteoblastic zone of bone tissue that contains mineralized type I collagen. 9,22,27,28While these four tissue regions differ compositionally, structurally they are continuous.There is a gradual increase in mineral content and decrease in collagenous ber organization during the transition from tendon into bone.The bers are also aligned and parallel at the tendon origin but become more curved, crossed and disorganized nearer the bone. 9This architecture provides a more rational stress distribution, enhances adherence strength and reduces risk of rupture or tearing 24 (Table 1).
0][31][32][33][34] In the initial stages of development, cartilage is mineralized to form bone through endochondral ossication, followed by brocartilage transition at the interface.6][37] During tendon formation and differentiation, Scleraxis (Scx) is an important transcription factor.][40] Experiments show Scx knockout leads to severe developmental abnormalities of insertions. 35It plays an important role in chondrogenesis through transcription, expression in proliferating chondrocytes, and differentiation of chondrocytes. 41,42OX9 remains active throughout early and late stages of chondrogenesis.With advancing development and differentiation, expressions of SOX9 and SCX gradually decrease regulated by various molecular drivers, including the transforming growth factor b (TGF-b) subfamily as a major factor since TGFb signaling controls progenitor cell behavior and is crucial for tendon and cartilage formation. 43TGF-b also modulates SCX expression, with lack of SCX leading to abnormal tendon and ligament development. 44In addition to TGF-b, bone morphogenetic protein (BMP) is another inuential factor.BMP-4 colocalizes with SCX in progenitor cells of bone protuberances and is regulated by SCX, inducing protuberances formation in the tendon-cartilage attachment region.The SCX/BMP-4 signaling transduction is necessary for these progenitor cells to differentiate into cartilage without the SCX/BMP-4 signaling transduction. 29,30It is thought that Indian hedgehog (Ihh) and parathyroid hormone-related protein (PTHrP) promote chondrocyte proliferation and differentiation, which act as a negative feedback loop in order to maintain the number and quality of chondrocytes. 21Research elucidated extracellular matrix expression during development with broblasts characteristically expressing type I collagen, collagen type II chondrocytes and collagen type X chondrocytes. 30,31

Irregularity of attachments into bone give mechanical integrity of enthesis
Provides an attachment site for tendons 1 (IGF-1), platelet-derived growth factor (PDGF), and transforming growth factor b (TGF-b). 22Getting tendon attachments healed requires TGF-1 and TGF-3, which are responsible for growth and differentiation of skeletal muscle. 52,535][56] When macrophages begin to secrete TGF-b1, the transition to the repair stage occurs, leading to broblast proliferation and scar tissue formation.Type III collagen is the main component of scar tissue. 55Although some degree of healing is achieved when tendon tissue is surgically xed to bone, physiologically normal tissues have different mechanical properties from repaired tissues, 57 even differing by orders of magnitude. 57Histologically, there are signicant differences between the healed tissue and the physiological state, with loss of continuity in collagen bers and no apparent gradient in mineral content, and the tissue at the healing interface consists of disorganized scar tissue. 58Damage to the interface between so tissue and bone is accompanied by bone loss, 58,59 making the repair of so tissue to bone more complex.The bone mineral density of rat rotator cuffs was signicantly reduced aer tendon injury and repair. 60imilar results were found in a study on canine distal phalanges, with decreased bone mineral density suggesting that bone resorption may be a contributing factor to the poor outcomes at the repair site. 59n the development of tendons and bones, mechanical loading plays a signicant role. 61Although the role of mechanobiology in the healing process is not yet clear, all cell types found near the attachment site have shown mechanical responsiveness. 62Current research suggests that cells are able to convert mechanical signals into gene regulation, which not only affects cell migration but also proliferation and differentiation. 63Additionally, the differences in the quantity of brocartilage found in different attachment sites may be related to mechanical forces. 64,65Muscle loading is essential for the growth and maturation of the attachment site, as evidenced by the reduction in mineral deposition and brocartilage formation observed when muscle loading is reduced using botulinum toxin, which affects postnatal attachment site maturation, 66 resulting in unorganized ber distribution and poorer mechanical properties. 67Several animal models have yielded similar results, with low-level loading (e.g., plaster xation) being optimal for healing. 57,68,69A rotator cuff injury animal model showed that plaster xation was more effective than exercise at promoting tendon healing. 57,68Similar results were found in a study comparing the effects of immediate and delayed mechanical loading on tendon-bone healing in an ACL model.Delayed loading led to greater healing than immediate or prolonged loading. 57,69In addition to mechanical stimuli, other physical factors such as electrical stimulation, ultraviolet radiation, and sound waves (e.g., ultrasound) have been studied.Through the stimulation of biological cascades, the increase of growth factors and cytokines levels, and the regulation of gene expression, they promote cell proliferation, differentiation, and osteogenesis.For example, low-intensity pulsed ultrasound (LIPUS) has been shown to increase levels of vascular endothelial growth factor (VEGF), thereby signicantly improving vascular distribution at the attachment site. 70,71ell phenotype and intercellular communication are another important inuencing factor in the regeneration of the attachment site.During the insertion of tendon into bone, tendons, brocartilages, mineralized brocartilages, and bone are present.Each tissue type exhibits its own cell phenotypes and matrix composition.Interactions between the three resident cell populations can be crucial for broblast, brochondrocyte, and osteoblast regeneration at the interface.Osteoblast-broblast interactions mediated by heterotypic cell interactions can contribute to attachment site regeneration, resulting in osteoblastic and/or broblastic transdifferentiation.The interactions may also lead to the differentiation of stem cells into brocartilage cells, thereby regenerating the interface between the cartilage and the brocartilage. 727][78] It is still unclear how intercellular communication works and what modulatory soluble factors are secreted in the healing environment, but intercellular communication encourages interface regeneration in both homing and healing environments.

Strategies for electrospinning to reconstruct special structures
Due to their high surface-to-volume ratios and interconnected porosities, berous materials have attracted a lot of attention from tissue engineering researchers, and diverse characteristics.Currently, there are several methods available for synthesizing nanober networks, including phase separation, template synthesis, and self-assembly. 79,80However, these methods have drawbacks such as long preparation time, limited ber length, and discontinuous ber scaffold structure. 81herefore, electrospinning technology stands out among many nanober fabrication methods due to its obvious advantages, including the ability to produce continuous ber networks relatively quickly and the versatility to work with various materials.Using a strong electric eld, electrospinning produces micro-and nanobers by jetting spinning from polymer melts or solutions.It is a novel processing method for producing nanoscale ultrane bers and can rapidly and costeffectively manufacture nonwoven ber structures. 82,83This ber formation technique overcomes surface tension of the solution by applying voltage, promoting the formation of Taylor cones, jetting streams that pass through the air, and stretching out bers with diameters ranging from nanometers to micrometers.These bers deposit on collectors with opposite charges or grounded. 84Currently, most tissue engineering scaffolds produced are relatively homogeneous and possess isotropic mechanical properties.One end of a tendon-bone repair scaffold should have uniaxially aligned nanobers, while the other end should have randomly oriented nanobers. 16,17dditionally, it should mimic the mineralization changes at the tendon-bone interface, with a structure that gradually increases in hardness from so to hard.It has been reported that electrospun polymer scaffolds with aligned bers can enhance stem cell differentiation, alignment, and collagen ber formation, [85][86][87][88][89][90] while randomly oriented bers play a role in osteogenesis and chondrogenesis. 91Although scaffolds with compositional, structural, and mechanical property gradients may have the potential to promote tendon-bone interface tissue growth, it is challenging to manufacture structures that transition from aligned bers to randomly oriented bers.Therefore, new manufacturing strategies are needed to prepare biomimetic scaffolds for interface tissue engineering (Fig. 3).
Over the past two decades, electrospinning has been positively explored for fabricating nanobers, enabling controllable preparation of nanober scaffolds with tunable composition, structure, alignment and functionality by modifying material choice, collector design, number of spinnerets, and electrospinning parameters.Typically, aligned nanobers are produced using a high-speed rotating drum or framed collectors with gap spacing between edges.Researchers have modulated collector design to control electrospun nanober structure, rendering them more suitable for repair of highly oriented tissues by achieving directional nanober alignment mimicking native microenvironment topography.For example, customized collector congurations such as patterned drums or arrays of conductive nanowires have enabled fabrication of anisotropically aligned nanober yarns or woven textiles for tendon/ligament regeneration.Overall, electrospinning offers a versatile nanobrous scaffold generation platform with tunable structural and biochemical cues to interface tendon/ligament repair.Xie et al. developed a collector consisting of two metal frames shaped like staplers, where nanoparticles were deposited in an aligned manner on the metal and a random manner in the gaps, 92 fabricating an "aligned-to-random" electrospun nanober scaffold.Using this scaffold, the tendon-bone insertion site could be modeled as collagen bers are structured.Studies showed this scaffold exhibited signicantly increased modulus and ultimate tensile strength.When cells were cultured on this scaffold, tendon broblasts in the aligned and random portions respectively demonstrated highly oriented and non-oriented morphologies, and tendon broblasts implanted in the random and aligned portions of the scaffold produced type I collagen rather than type II collagen. 92This suggests broblasts cultured in this system could generate an appropriate ECM for tendon repair.Fig. 4A and B demonstrates the experimental setup for fabricating a typical "ordered and disordered" electrospun nanober scaffold.Kishan et al. implemented uniform brous mat collection via custom collectors equipped with synchronized rotation and utilized periodic copper wires to guide directional ber alignment (Fig. 4C).Biodegradable polyurethane (BPUR) at varying contents were electrospun to fabricate composition gradient mesh scaffolds with and without ber orientation for tendon-bone graing. 93The polymer gradient from BPUR50 to BPUR10 along the aligned direction allowed progressive transition in mechanical properties. 93In addition to allowing continuous gradient in polymer content, the technique can be used to generate activity gradients along the arrangement direction by adding additives into the polymer solution.Neither the compositional gradients nor ber alignment of the scaffolds affected cell attachment, and meshes with different alignments exhibited signicant stiffness gradients.Both studies employed gap electrospinning for scaffold preparation, requiring almost no additional equipment beyond standard electrospinning apparatus, representing an economical and convenient approach achieving unique microstructural control.However, the method is limited in fabricating scaffolds of large sizes and thicknesses. 96,97In this conguration, the ordered and disordered nanober regions mimic the graded tendon-to-bone transition.Such modulated nanober topography could guide cell behaviors relevant to regenerating the zonal interfaces.
Researchers from Tindell et al. demonstrated that magnetically assisted electrospinning allows for ne spatial control over ber alignments, resulting in wavy interfaces between aligned and random ber regions. 94Ajao et al. employed magnetically assisted electrospinning technique using additional cylindrical magnets where well-oriented nanobers were observed at magnet tops while non-aligned at other positions 98 (Fig. 4D and  E).By adjusting magnet congurations, magnetically assisted electrospinning can achieve various ber gradients including random, multi-directional and other complex gradients.This method only requires minor modications to conventional electrospinning setup via addition of an array of magnets of different types and congurations to spatially control ber alignments and generate desired ber architectures.An advantage of this general device is its ability to be congured in a modular manner without changing the original setup.Researchers also employed auxiliary electrodes to inuence electrospinning jet trajectory and thereby control deposition regions and direction of electrospun bers. 99,100Magnetically and electrically assisted elds provide technological basis for fabrication of structurally graded scaffolds needed for tendon- bone interface regeneration.In summary, these studies demonstrated modulated ber alignments and compositional gradients mimicking tendon-bone transition using simple yet effective electrospinning modications, holding promise for interface regeneration applications.The techniques of magnetically or electrically assisted electrospinning allow spatial control over ber deposition enabling generation of bers with gradients to interface repair.In addition to the aforementioned enhancements made directly on electrospinning equipment, post-processing methods on electrospun bers can also fabricate scaffolds meeting interface tissue engineering requirements.Manufacturing post-treatment methods can improve orientation by converting poorly aligned bers into highly oriented meshes via combined application of tensile stretching and thermal annealing. 101,102Zong et al. rst described a post-drawing method, investigating the microstructure, morphology and texture of electrospun poly(lactic-coglycolic acid) non-woven membranes aer stretching and heat treatment, as well as their degradation and mechanical properties.Results showed the stretched and annealed membranes had higher crystallinity and evident lamellar structuring with improved orientation.Material orientation and tensile strength both increased with rising draw ratios. 103Post-drawing provides a viable alternative to obtaining highly aligned bers or increasing orientation from randomly oriented meshes, with signicantly enhanced mechanical properties.However, drawbacks exist such as ∼20% reduction in microstructural porosity post-stretching. 103Unlike post-drawing, Yu et al. employed photothermal welding technique on aligned ber scaffolds to successfully fabricate gradients from alignment to randomness. 104They premixed ICG directly into the PU solution for electrospinning using a drum-type collector to generate uniaxially aligned nanobers.Post fabrication, laser irradiation activated the photothermal material (ICG) to generate heat, melting the nanobers at their melting points and welding them.The photothermally-induced structural changes in nanober scaffolds satised structural gradation, with subsequent graded mineralization mimicking biological gradients.In vitro studies showed the scaffold was biocompatible and guided tendon stem cell morphological elongation and tenogenic and osteogenic differentiation, with in vivo investigations via immunohistochemical and biomechanical analyses conrming improved rabbit supraspinatus injury healing.To achieve aligned-to-random orientation in electrospun nanober membranes, researchers have also employed stacking of multiple electrospun ber layers to induce orientation transition effects, 95 as depicted in Fig. 4F where Cong et al. separately fabricated membranes including aligned nanobers PCL (aPCL), random PCL (nPCL), aPCL-collagen I, nPCL-collagen II and nPCL-nanohydroxyapatite (nHA) bers.They layerelectrospun nPCL-collagen II and nPCL-nHA onto one end of aPCL-collagen I in a stepwise manner.By interlayering Col I, Col II and nHA onto PCL scaffolds, they simulated bone gras and conducted prosthetic reconstruction.Results showed scaffolds with good biocompatibility, with signicantly more neo-brocartilage formation observed at experimental group bone-gra junctions versus controls, indicating improved bone ingrowth, larger brocartilage formation and better biomechanical properties.In addition to the above-mentioned methods of fabricating nanober scaffolds with aligned-Fig.4 (A) Stapler-like device for manufacturing nanofiber scaffolds and (B) SEM images of randomly arranged and uniaxial nanofiber scaffolds were obtained.Inset: schematic illustration of electric field lines between needle and collector.(C) Electrospinning device with custom rotating collector for fabricating component gradients and SEM images of fibers.(D) Electrospinning apparatus side view, and (E) collector equipped with a single magnet.][94][95] random orientation gradients via various improved apparatus, some researchers have incorporated various active components into conventionally aligned orientation or random orientation ber membranes to aim at promoting tendon-bone healing.We will discuss these related studies in more detail in the next section (Table 2).

Strategies for preparing polymers by electrospinning
The selection of biomaterials plays a crucial role in determining the key parameters of tissue engineering scaffolds, such as structure, biochemical properties, and mechanical performance.However, it is still unclear which biomaterial is the optimal choice for generating such scaffolds.It has been proven that bone-to-tendon tissue engineering scaffolds can be constructed from a variety of biomaterials.0][111] Because PLGA was approved by the U.S. Food and Drug Administration (FDA), it has gained widespread attention as a scaffold for tissue engineering and drug delivery. 109][116] Currently, the primary focus has been on copolymers, blends or polymer-ceramic/bioactive glass composites, aimed at fully leveraging the properties of different materials.Composite strategies attract signicant interest in interface tissue engineering as incorporating different materials can better mimic the graded structures of native attachments and exert distinct biological effects.Liu et al. employed electrospinning to generate scaffolds with gradient concentration of PLGA and PCL, demonstrating the ability to recreate the tendon-bone microenvironment and reconstruct the interface. 108Evaluation of the scaffolds' induction of adipose-derived mesenchymal stem cells (ASCs) osteogenic differentiation showed the gradient in mineral content on the nanober surfaces could guide graded ASC differentiation into osteoblasts for rotator cuff repair. 108Naghashzargar et al. fabricated a novel scaffold material using silk broin as the core encapsulated by P3HB and PCL bers via electrospinning.Results indicated this material possessed enhanced tensile strength meeting the mechanical demands for tendon-bone reconstruction, and demonstrated good biocompatibility in L929 cell studies. 117one loss frequently accompanies tendon-bone injuries, and incorporating bone bioactive materials is a common polymer strategy to enhance electrospun scaffold osteogenic activity.These include calcium phosphate ceramics (particularly hydroxyapatite (HA) 118,119 and b-tricalcium phosphate (b-TCP) 105,120 ), bioactive glasses (mainly silicate-based 45S5 Bio-glass® 121,122 ).Lv et al. incorporated hydroxyapatite (HA) into poly(lactic acid) (PLLA) to fabricate electrospun nanober membranes as rotator cuff repair patches.The results showed hydrophilicity of electrospun bers improved with good cytocompatibility, enhancing alkaline phosphatase (ALP) expression of rat bone marrow mesenchymal stem cells (BMSCs), indicating better induction of rat BMSC osteogenesis. 123Soo Kim et al. generated a tendon-to-bone structural scaffold consisting of 4 layerscollagen forms the tendon layer, brocartilage bonds to chondroitin sulfate to form brocartilage, HA forms the mineralized brocartilage, and calcium forms the bone layer.Young's modulus increased while elongation decreased. 124Further analysis showed that tenocytes, chondrocytes, and osteoblasts throughout the tendon and brocartilage layers, as well as osteoblasts in the bone layer, showed enhanced proliferation. 124Erisken et al. 125 fabricated b-TCP graded electrospun PCL nanober membranes and analyzed their mechanical properties.During tensile deformation, Young's modulus increased gradually along the scaffold with increasing mineral content, whereas elongation decreased. 125,126urthermore, MC3T3 pre-osteoblastic cell attachment and migration showed different behaviors and morphologies along the scaffold. 125,126According to these studies, structural and chemical gradients affect mechanical properties and cellular behavior of scaffolds, especially mineral gradients.Biological factors like TGF-b3 may also be important promotors in the rotator cuff tear healing process.Reifenrath et al. studied a TGF-b3 loaded electrospun shellac-graedpolycaprolactone (CS-g-PCL) ber scaffold and compared its biomechanical and histological effects on tendon healing with unloaded ber scaffolds in a rat chronic tendon defect model.The results showed the ber scaffold with better degradability and biocompatibility, while brosis appeared reduced as a foreign body encapsulation and scar formation indicator. 127ao et al. synthesized strontium-doped mesoporous bioactive glass nanoparticles (Sr-MBG) via a sol-gel method and prepared a biphasic inductive and immunomodulatory electrospun brous scaffold containing Sr-MBG (BIIEFS).Mesenchymal stem cells (MSCs) were shown to differentiate osteogenically and chondrogenically when treated with BIIEFS, with multiple bioactive ions being released, and macrophages were conrmed to acquire an M2 phenotype when stimulated with BIIEFS.Experimental results showed that electrospun scaffolds increased the number of M2 macrophages, while synchronous regeneration of tendon, brocartilage, and bone was observed, which signicantly enhanced supraspinatus tendon-humerus biomechanical strength. 128hese studies exemplify how incorporating bioactive factors and materials into electrospun scaffolds holds promise for developing clinically translatable therapeutics through regulating the wound microenvironment and cellular responses during interface tissue regeneration.Continued optimizations modulating scaffold composition, topography and biomechanical integrity are important next steps towards achieving functional restoration.Metal ions play important roles in tissue repair and have wide clinical applications.However, their use is limited due to narrow metabolic and healing windows.In this study, Yang et al. produced biphasic metal-exible electrospun ber membranes from a MOF carrier through continuous electrospinning and matching the longitudinal spatial morphology of multiple tissues simultaneously. 129As a carrier, MOF not only enables sustained metal ion release but also promotes osteogenesis and tenogenesis on the scaffold.Studies showed this layered electrospun brous structure could accelerate tenogenesis, biological mineralization and vascularization.During in vivo validation, the agent was shown to promote tendon and bone tissue repair, as well as brocartilage reconstruction, facilitating synchronized regeneration of several tissues at the injured tendon-bone interface.In summary, Using MOFs as the base, electrospun ber membranes will be biphasic metal-exible represent a novel class of biodegradable so scaffolds with tremendous potential for reconstructing tissue defects, especially graded tissue injuries. 129These studies exemplify how incorporating bioactive factors and materials into electrospun scaffolds holds promise for developing clinically translatable therapeutics through regulating the wound microenvironment and cellular responses during interface tissue regeneration.Continued optimizations modulating scaffold composition, topography and biomechanical integrity are important next steps towards achieving functional restoration.There is also a kind of acellular matrix materials used as interface tissue engineering to promote tendon and bone healing, but this review mainly focuses on the materials and applications of electrospinning technology, so it will not be discussed (Table 3).

Evaluation and analysis of mechanical properties of bionic scaffolds
Since the function of the BTI interface is to bear and transmit loads between mechanically different tissues, it must be able to withstand certain mechanical loads.Evidence also suggests that cells near the interface can sense mechanical forces, which, through genetic regulation, further inuence cell migration, extracellular matrix adhesion, and cell proliferation and differentiation. 21Therefore, excellent mechanical strength is a necessary condition for the fabricated bionic scaffold.In this section, we will discuss the mechanical strength of some of the electrospun bionic scaffolds mentioned above.
In the study by Xie et al., 92 tensile tests were used to evaluate the mechanical properties of the scaffold.The scaffold was tested on a tensile testing machine to measure its tensile strength and elastic modulus.Aligned bers: tensile strength reached approximately 15 MPa, and elastic modulus was 100 MPa.Random bers: tensile strength was about 5 MPa, and elastic modulus was 50 MPa.The tensile strength of the scaffold depends on the arrangement of the bers.The tensile strength of the aligned bers was signicantly higher than that of the random bers.In the study by Kishan et al., 93 various testing  4.These studies demonstrate the importance of mechanical properties for tissue engineering scaffolds.In summary, these studies found that aligned bers generally have superior tensile strength and elastic modulus compared to random bers.However, considering the complex structural variations of the BTI interface, the quality of a bionic scaffold cannot be judged solely by its mechanical strength.

Summary and future perspectives
As part of this article, the author summarizes how electrospinning technology can be used in the design of tendon-bone interface tissues.Various cell types maintain stability within the tendon-bone interface, which enables efficient transmission of muscular forces to the skeleton.The mechanism of tendon-bone interface healing is complex yet crucial for patient prognosis, and current treatments still fall short in achieving complete reconstruction of the tendon-bone interface.The author summarizes the research on the biological and mechanical mechanisms of tendon-bone interface healing and discusses the developmental characteristics and healing mechanisms of injured interfaces, which primarily involve molecular biology, physical factor stimulation, and mechanical stimulation.The article also delves into the strategies and materials involved in scaffold preparation for bone-tendon interface tissue engineering using electrospinning technology.Tissue engineering to achieve regeneration of muscle-tendon/ligament-bone interfaces presents an attractive strategy for providing functional transplants and improving clinical outcomes following injuries.In spite of this, attachment site tissue engineering represents a signicant challenge for biologists and engineers because of the complex structure and the critical interdependences between structure, function, and mechanical properties.Tissue engineering has made rapid advances in recent years, but functional attachment site-like tissues have yet to be created.In order to improve the quality of attachment sites, a deeper understanding of their structure-

Fig. 1
Fig. 1 Common anatomical locations of fibrocartilage insertions in the human body and a schematic diagram illustrating the transitional zones within the insertion site.10

Fig. 2 (
Fig. 2 (A) Schematic diagram illustrating the structural and compositional changes within the transitional zones at the tendon-bone insertion site. 21(B) Schematic diagram of an histopathological section of the supraspinatus tendon-bone insertion in mice.The Alcian blue staining of proteoglycans in tendon, fibrocartilage, and mineralized fibrocartilage shows the presence of proteoglycans, highlighting the compositional gradient characteristic of the insertion site.10

Fig. 3
Fig. 3 Schematic representation of manufacturing strategies for biomimetic scaffolds in interface tissue engineering.

Fig. 5
Fig. 5 Summary diagram of interface tissue engineering scaffold materials prepared by electrospinning.

Table 2
Summary of methods for fabricating aligned-to-random scaffold

Table 3
Summary of electrospun polymer strategies for tendon-bone interface tissue engineering The tensile strength of the aligned ber scaffold was 23.5 ± 1.2 MPa.The tensile strength of the random ber scaffold was 12.8 ± 0.9 MPa.The elastic modulus of the aligned ber scaffold was 162 ± 10 MPa.The elastic modulus of the random ber scaffold was 92 ± 8 MPa.Elongation at break: the elongation at break of the aligned ber scaffold was 21.7 ± 1.8%.The elongation at break of the random ber scaffold was 15.3 ± 1.4%.Similarly, the elastic modulus of the aligned bers was higher than that of the random bers, indicating that aligned bers provide stronger mechanical support and stability.Similarly, other studies have recorded tensile strengths ranging from 12 to 45 MPa and elastic moduli from 90 to 220 MPa.Specic data are summarized in Table function relationship, as well as mechanisms of attachment site development, homeostasis, and regeneration, is necessary.Biomechanical and biological factors drive the development of naturally occurring insertion sites, which are functionally graded tissues composed of multiple cells types and extracellular matrix components.Transcription factors specic to different types of cells initiate the development of graded interfaces between bone and muscle-tendon or ligament.Consequently, muscle strength signals determine the maturity and growth of the attachment site.As such, fundamental research on the molecular and mechanical factors regulating natural attachment site development and homeostasis is essential for advancing future interface tissue engineering approaches.The interface between so tissues and bones plays an important role in musculoskeletal function, and its regeneration through tissue engineering promises to improve clinical results.However, there are still several challenges to overcome before the application of current research in clinical practice: (1) regarding the direction of mechanism research, although there have been numerous studies on the molecular mechanisms of tendon-bone interface healing, the core keys to healing remain unclear.(2)Interms of experimental translation, interdisciplinary collaboration is required to demonstrate the classication, composition, and implantation methods of various scaffold materials, aiming to obtain higher-level clinical

Table 4
Summary of mechanical properties of bionic scaffolds and nd the most suitable treatment options.(3) Alongside the emergence of new technologies and materials, ethical concerns have also arisen.Complex tissue engineering implants and the introduction of exogenous cells pose risks to patients.Therefore, close attention to ethical issues is still necessary before implementing these treatment strategies. research